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PMID: 17507929

Chendrimada TP et al
Nature 2007 Jun 14;447(7146):823-8
MicroRNA silencing through RISC recruitment of eIF6.

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Conclusion arose from an incorrect premise regarding eIF6

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BioMed Crit Comm 2008; 1:1269
Posted: Nov 18, 2008 CCID: 1269
Commentator: rrmari	Ave. score of this commentator: 2.3 for 73 scored comments.

The authors found that depletion of eIF6 abrogated the otherwise expected inhibition of translation by miRNAs. Presuming that eIF6 was involved in regulating translation initiation, this would seem to implicate miRNAs in controlling translation initiation. According to a discussion by M Kozak (PMID: 18692553), however, this premise is probably wrong, and "eIF6 is probably not a translation initiation factor", based on experiments in yeast in which eIF6 was not required for translation initiation but instead was required for pre-rRNA processing and biogenesis of the 60S ribosomal subunit (PMID: 11238882, PMID: 9891075). Deficits in translation initiation are but a very indirect effect when ribosome biogenesis is impaired. Kozak noted that the only eIF6-dependent function of lin-4 miRNA observed by the authors in worms was degradation of the mRNA, but no effects were observed in vivo at the level of translation per se.

Key authors in this publication:

Chendrimada TP, Finn KJ, Ji X, Liebhaber SA, Pasquinelli AE, Shiekhattar R.

View Linked Comments	PMID: 17507929
Chendrimada TP et al Nature 2007 Jun 14;447(7146):823-8 MicroRNA silencing through RISC recruitment of eIF6.