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PMID: 19092803

Kaplan N et al
Nature 2009 Mar 19;458(7236):362-6
The DNA-encoded nucleosome organization of a eukaryotic genome.

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Ignored discrepancy with earlier, more relevant, database

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BioMed Crit Comm 2010; 3:2269
Posted: Dec 21, 2010 CCID: 2269
Commentator: Anonymous	Ave. score of anonymous comments: 2.4 for 548 scored comments.

As noted by Locke et al (PMID: 21084631), Kaplan's in vitro dataset was derived from an experiment using a 0.4:1 mass ratio of histone to DNA. A more natural ratio of 1:1, corresponding to actual levels in vivo, was used by a previous report by Zhang et al (PMID: 19620965), also concerning eukaryotic chromatin studied in vitro. The correlation between the two databases was rather low (r = 0.69). Because the Zhang publication appeared about ten weeks prior to the submission of Kaplan's manuscript to Nature, Kaplan could have noted this discrepancy for consideration by their readers.

Key authors in this publication:

Fondufe-Mittendorf Y, Kaplan N, Lieb JD, Moore IK, Segal E, Widom J.

View Linked Comments	PMID: 19092803
Kaplan N et al Nature 2009 Mar 19;458(7236):362-6 The DNA-encoded nucleosome organization of a eukaryotic genome.